PACT/PRKRA and p53 regulate transcriptional activity of DMRT1

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dc.contributor.authorFujitani, Kazuko
dc.contributor.authorOtomo, Asako
dc.contributor.authorNagayama, Yuto
dc.contributor.authorTachibana, Taro
dc.contributor.authorKato, Rika
dc.contributor.authorKawashima, Yusuke
dc.contributor.authorKodera, Yoshio
dc.contributor.authorKato, Tomoko
dc.contributor.authorTakada, Shuji
dc.contributor.authorTamura, Kei
dc.contributor.authorTakamatsu, Nobuhiko
dc.contributor.authorIto, Michihiko
dc.date.accessioned2024-05-14T15:27:46Z
dc.date.available2024-05-14T15:27:46Z
dc.date.issued2020
dc.description.abstractAbstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate.
dc.identifier.doi10.1590/1678-4685-gmb-2019-0017
dc.identifier.issn1415-4757
dc.identifier.otherS1415-47572020000400702-scl
dc.identifier.urihttps://repositorio-aptaregional.agricultura.sp.gov.br/handle/123456789/905
dc.relation.ispartofGenetics and Molecular Biology
dc.subjectp53
dc.subjectDMRT1
dc.subjectgermline stem cell
dc.subjecttestis
dc.subjectXenopus
dc.titlePACT/PRKRA and p53 regulate transcriptional activity of DMRT1
dc.typeArtigos
oaire.citation.issue2
oaire.citation.volume43

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