PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil

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dc.contributor.authorSilveira, Ana Paula Stefanello da
dc.contributor.authorVieira, Victor Bruno Duarte
dc.contributor.authorBatalini, Leticia Surian
dc.contributor.authorCarmo, Silvia Barbosa do
dc.contributor.authorFriozi, Elisabete
dc.contributor.authorArruda, Eduardo José de
dc.contributor.authorLima Junior, Manoel Sebastião da Costa
dc.contributor.authorNeitzke-Abreu, Herintha Coeto
dc.date.accessioned2024-05-16T13:47:12Z
dc.date.available2024-05-16T13:47:12Z
dc.date.issued2018
dc.description.abstractAbstract INTRODUCTION Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized. METHODS PCR and parasitological tests were conducted. RESULTS PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples. CONCLUSIONS Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.
dc.identifier.doi10.1590/0037-8682-0040-2018
dc.identifier.issn0037-8682
dc.identifier.otherS0037-86822018000600843-scl
dc.identifier.urihttps://repositorio-aptaregional.agricultura.sp.gov.br/handle/123456789/1605
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical
dc.subjectCanine visceral leishmaniasis
dc.subjectPCR
dc.subjectSensitivity
dc.subjectPeripheral blood
dc.titlePCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil
dc.typeArtigos
oaire.citation.endPage847
oaire.citation.issue6
oaire.citation.startPage843
oaire.citation.volume51

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