Microscale extraction method for HPLC carotenoid analysis in vegetable matrices

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dc.contributor.authorPacheco, Sidney
dc.contributor.authorPeixoto, Fernanda Marques
dc.contributor.authorBorguini, Renata Galhardo
dc.contributor.authorNascimento, Luzimar da Silva de Mattos do
dc.contributor.authorBobeda, Claudio Roberto Ribeiro
dc.contributor.authorSantiago, Manuela Cristina Pessanha de Araújo
dc.contributor.authorGodoy, Ronoel Luiz de Oliveira
dc.date.accessioned2024-05-15T13:16:18Z
dc.date.available2024-05-15T13:16:18Z
dc.date.issued2014
dc.description.abstractIn order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L.), carrot (Daucus carota L.), sweet potato with orange pulp (Ipomoea batatas (L.) Lam.), pumpkin (Cucurbita moschata Duch.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and sweet potato (Ipomoea batatas (L.) Lam.) flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories.
dc.identifier.doi10.1590/0103-9016-2013-0402
dc.identifier.issn0103-9016
dc.identifier.otherS0103-90162014000500011-scl
dc.identifier.urihttps://repositorio-aptaregional.agricultura.sp.gov.br/handle/123456789/1222
dc.relation.ispartofScientia Agricola
dc.subjectβ-carotene
dc.subjectα-carotene
dc.subjectlycopene
dc.titleMicroscale extraction method for HPLC carotenoid analysis in vegetable matrices
dc.typeArtigos
oaire.citation.endPage419
oaire.citation.issue5
oaire.citation.startPage416
oaire.citation.volume71

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