Full confluency, serum starvation, and roscovitine for inducing arrest in the G0/G1 phase of the cell cycle in puma skin-derived fibroblast lines

Página do item simplificado
dc.contributor.authorRodrigues, Luanna Lorenna Vieira
dc.contributor.authorMoura, Yasmin Beatriz França
dc.contributor.authorViana, João Vitor da Silva
dc.contributor.authorOliveira, Lhara Ricarliany Medeiros de
dc.contributor.authorPraxedes, Érika Almeida
dc.contributor.authorVieira Neto, José de Brito
dc.contributor.authorSales, Sarah Leyenne Alves
dc.contributor.authorSilva, Herlon Victor Rodrigues
dc.contributor.authorLuciano, Maria Claudia dos Santos
dc.contributor.authorPessoa, Claudia
dc.contributor.authorPereira, Alexsandra Fernandes
dc.date.accessioned2024-05-16T11:01:03Z
dc.date.available2024-05-16T11:01:03Z
dc.date.issued2023
dc.description.abstractAbstract The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of the factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We evaluated the effects of full confluency (~100%), serum starvation (0.5% serum), and roscovitine (15 µM) treatments on the cell cycle synchronization in G0/G1 of puma skin-derived fibroblasts by flow cytometric analysis. Also, we assessed the effects of these synchronization methods on morphology, viability, and apoptosis levels using microscopy tools. The results showed that culturing the cells to confluence for 24 h (84.0%), 48 h (84.6%), and 72 h (84.2%) and serum starvation for 96 h (85.4%) yielded a significantly higher percentage of cells arrested in the G0/G1 (P < 0.05) phase than cells not subjected to any cell cycle synchronization method (73.9%). Nevertheless, while serum starvation reduced the percentage of viable cells, no difference was observed for the full confluence and roscovitine treatments (P > 0.05). Moreover, roscovitine for 12 h (78.6%) and 24 h (82.1%) was unable to synchronize cells in G0/G1 (P > 0.05). In summary, full confluency induces puma fibroblast cell cycle synchronization at the G0/G1 stage without affecting cell viability. These outcomes may be valuable for planning donor cells for somatic cell nuclear transfer in pumas.
dc.identifier.doi10.1590/1984-3143-ar2023-0017
dc.identifier.issn1984-3143
dc.identifier.otherS1984-31432023000100210-scl
dc.identifier.urihttps://repositorio-aptaregional.agricultura.sp.gov.br/handle/123456789/1547
dc.relation.ispartofAnimal Reproduction
dc.subjectfelids
dc.subjectcell cycle synchronization
dc.subjectculture to confluence
dc.subjectsomatic cell nuclear transfer
dc.titleFull confluency, serum starvation, and roscovitine for inducing arrest in the G0/G1 phase of the cell cycle in puma skin-derived fibroblast lines
dc.typeArtigos
oaire.citation.issue1
oaire.citation.volume20

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed to upon submission
Descrição:
Baixar

Coleções

Artigos