Navegando por Autor "Aguiar, Daniel Moura"

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    Bartonella species pathogenic for humans infect pets, free-ranging wild mammals and their ectoparasites in the Caatinga biome, Northeastern Brazil: a serological and molecular study
    (2017) Fontalvo, Mariana Campos; Favacho, Alexsandra Rodrigues de Mendonça; Araujo, Andreina de Carvalho; Santos, Naylla Mayana dos; Oliveira, Glauber Meneses Barboza de; Aguiar, Daniel Moura; Lemos, Elba Regina Sampaio de; Horta, Mauricio Claudio
    ABSTRACT This study verified the occurrence of Bartonella spp. in dogs, cats, wild mammals and their ectoparasites in Petrolina and Lagoa Grande Counties, Pernambuco, located in a semi-arid region in Northeastern Brazil. Anti-Bartonella spp. antibodies were detected by indirect immunofluorescence assay (IFA) in 24.8% of dogs (27/109) and in 15% of cats (6/40). Bartonella sp. DNA was identified by PCR performed on DNA extracted from blood and ectoparasites using primers targeting Bartonella sp. gltA and ribC genes in 100% (9/9) of Pulex irritans from Cerdocyon thous, 57.4% (35/61) of P. irritans from dogs, 2.3% (1/43) of Ctenocephalides felis felis from dogs, 53.3% (24/45) of C. felis felis from cats, and 10% (1/10) of Polyplax spp. from Thrichomys apereoides. DNA sequencing identified Bartonella clarridgeiae and Bartonella henselae in C. felis felis from cats, Bartonella rochalimae in P. irritans from dog and C. thous, and Bartonella vinsoni berkhofii in P. irritans from dog.
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    Bartonella species pathogenic for humans infect pets, free-ranging wild mammals and their ectoparasites in the Caatinga biome, Northeastern Brazil: a serological and molecular study
    (2017) Fontalvo, Mariana Campos; Favacho, Alexsandra Rodrigues de Mendonça; Araujo, Andreina de Carvalho; Santos, Naylla Mayana dos; Oliveira, Glauber Meneses Barboza de; Aguiar, Daniel Moura; Lemos, Elba Regina Sampaio de; Horta, Mauricio Claudio
    ABSTRACT This study verified the occurrence of Bartonella spp. in dogs, cats, wild mammals and their ectoparasites in Petrolina and Lagoa Grande Counties, Pernambuco, located in a semi-arid region in Northeastern Brazil. Anti-Bartonella spp. antibodies were detected by indirect immunofluorescence assay (IFA) in 24.8% of dogs (27/109) and in 15% of cats (6/40). Bartonella sp. DNA was identified by PCR performed on DNA extracted from blood and ectoparasites using primers targeting Bartonella sp. gltA and ribC genes in 100% (9/9) of Pulex irritans from Cerdocyon thous, 57.4% (35/61) of P. irritans from dogs, 2.3% (1/43) of Ctenocephalides felis felis from dogs, 53.3% (24/45) of C. felis felis from cats, and 10% (1/10) of Polyplax spp. from Thrichomys apereoides. DNA sequencing identified Bartonella clarridgeiae and Bartonella henselae in C. felis felis from cats, Bartonella rochalimae in P. irritans from dog and C. thous, and Bartonella vinsoni berkhofii in P. irritans from dog.
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    Risk factor analysis associated with Neospora caninum in dairy cattle in Western Brazilian Amazon
    (2021) Venturoso, Poliana de Jesus Souza; Venturoso, Osvaldo Juliatti; Silva, Gisele Glomba; Maia, Maerle Oliveira; Witter, Rute; Aguiar, Daniel Moura; Pacheco, Richard de Campos; Ferreira, Elvino; Costa, Alvimar José da; Santos-Doni, Thais Rabelo dos
    Abstract Neospora caninum is considered to be one of the main causes of abortion among cattle. The present survey was conducted in the municipality of Rolim de Moura, Rondônia State, Brazil. A questionnaire that investigates the epidemiological aspects of neosporosis was used in the analysis of risk factors associated with the animal-level and herd-level prevalence in dairy cattle. A total of 416 bovine blood samples were collected from 30 farms, and N. caninum antibody levels were measured by Indirect Fluorescent Antibody Test (IFAT). Analysis of dairy cattle serum samples revealed the presence of anti-N. caninum antibodies to be 47.36% (n = 197). Risk factors associated with N. caninum infection were the management system and access locations of dogs. The results of the present survey indicated that infection of dairy cattle with N. caninum is widespread in the studied region of Western Amazon, which has implications for prevention and control of neosporosis in this region. Therefore, integrated control strategies and measures are recommended to prevent and control N. caninum infection in dairy cattle. In addition, direct contact between dairy cattle, dogs and wild animals, which can influence the epidemiology of neosporosis, should be investigated further. Resumo A infecção por Neospora caninum é considerada uma das principais causas de aborto entre bovinos. Esta pesquisa foi realizada no município de Rolim de Moura, estado de Rondônia, Brasil. Um questionário que investiga os aspectos epidemiológicos da neosporose foi utilizado na análise dos fatores de risco associados à prevalência em animais e em rebanhos. Um total de 416 amostras de sangue bovino foi colhido em 30 fazendas, e os níveis de anticorpos de N. caninum foram mensurados pela reação de Imunofluorescência Indireta (RIFI). A análise das amostras mostrou prevalência de anticorpos contra N. caninum de 47,36% (n = 197). Os fatores de risco associados à infecção por N. caninum foram o sistema de manejo e os locais de acesso dos cães. Os resultados da presente pesquisa indicam que a infecção de bovinos leiteiros com N. caninum está disseminada na região estudada da Amazônia Ocidental, o que tem implicações para a prevenção e controle da neosporose nessa região. Portanto, estratégias e medidas de controle integrado são recomendadas para prevenir e controlar a infecção por N. caninum em gado leiteiro. Além disso, o contato íntimo entre gado leiteiro, cães e animais selvagens, pode influenciar a epidemiologia da neosporose e deve ser investigada mais detalhadamente.